4 And there were more chiefs among the sons of Eleazar than among the sons of Ithamar; and this is how they were grouped: of the sons of Eleazar there were sixteen, all heads of families; and of the sons of Ithamar, heads of families, there were eight.
5 So they were put into groups, by the Lord's decision, one with another; for there were rulers of the holy place and rulers of the house of God among the sons of Eleazar and the sons of Ithamar.
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Commentary on 1 Chronicles 24 Matthew Henry Commentary
Chapter 24
This chapter gives us a more particular account of the distribution of the priests and Levites into their respective classes, for the more regular discharge of the duties of their offices, according to their families.
1Ch 24:1-19
The particular account of these establishments is of little use to us now; but, when Ezra published it, it was of great use to direct their church affairs after their return from captivity into the old channel again. The title of this record we have v. 1-These are the divisions of the sons of Aaron, not by which they divided one from another, or were at variance one with another (it is a pity there should ever be any such divisions among the sons of Israel, but especially among the sons of Aaron), but the distribution of them in order to the dividing of their work among themselves; it was a division which God made, and was made for him.
1Ch 24:20-31
Most of the Levites here named were mentioned before, ch. 23:16, etc. They were of those who were to attend the priests in the service of the house of God. But they are here mentioned again as heads of the twenty-four courses of Levites (and about so many are here named), who were to attend the twenty-four courses of the priests: they are therefore said to cast lots over against their brethren (so they are called, not their lords), the sons of Aaron, who were not to lord it over God's clergy, as the original word is, 1 Pt. 5:3. And, that the whole disposal of the affair might be of the Lord, the principal fathers cast lots over against their younger brethren; that is, those that were of the elder house came upon the level with those of the younger families, and took their place, not by seniority, but as God by the lot directed. Note, In Christ no difference is made between bond and free, elder and younger. The younger brethren, if they be faithful and sincere, shall be no less acceptable to Christ than the principal fathers.